... choice in assays ranging from use as a reporter gene in vitro and in vivo to use in biosensors. The most...effect of mutations of these regulatory elements on gene expression. Empty backbones for inserting regulatory...an intron in the middle of the Firefly luciferase gene.. TREAT (3( T hree)′- R NA E nd A ccumulation during...into which you can clone your regulatory element or gene of interest into to create a luciferase reporter... Firefly/Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of...enhancer regions. Mutations within the luciferase gene allow for increased copy number in E. coli and expression...cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc ...

... for gene expression GFP Varies pBABE GFP - Retroviral gene expression pMKO.1...for mammalian gene silencing pLVTHM - lentiviral plasmid for mammalian gene silencing ...high expression of UAS-driven transgenes Worm unc-54, variety of worm gene promoters C. elegans vector ... nematode transgenesis Rosenberg Lab Plasmids - Transgenic single-copy gene ...part, we will assume that you want to express a gene; however, we have a section at the end for if ...If you want to drive expression of your favorite gene, you will need a plasmid with a promoter that...hybrid plasmids Fly UAS, MT Targeted Gene Expression - Rubin lab plasmids...

...Gibson none S. pyogenes Zack pHL-H1-ccdB-mEF1a-RiH 60601 Mammalian BamHI/EcoRI none S. pyogenes Hygro Hotta...none S. pyogenes Mark pLenti-sgRNA-Lib 53121 Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5...are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into...capable of simultaneously repressing multiple target genes in Escherichia coli. Koffas Do you have suggestions...BsaI none S. pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol...none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein...elegans BsaI none S. pyogenes Joung pCFD3-dU6:3gRNA 49410 Drosophila BbsI none S. pyogenes Virmilion Bullock...