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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18 results for: codon optimized

Showing: 1 - 18 of 18 results

  1. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Resources This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of ...fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based...Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1...new window) Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ... contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR ..., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via the links...from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its ... contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...contains a single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected ...contains a single cassette expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter...

  3. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...recombination of the DNA between target sites. Codon optimization: Modifying the DNA sequence of the recombinase...integration efficiency but research groups continue to optimize these tools. The best characterized SSR systems...inducible systems, you can precisely control and optimize expression of your gene of interest. Inducible...recombinase to align with your organism's preferred codon usage will improve expression. For example, iCre... expression strategies. Gene Expression: A stop codon flanked with loxP sites (often called a "lox-STOP-lox...absence of Cre. In the presence of Cre, the stop codon is excised, and gene expression proceeds. Gene Knockout... Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101...

  4. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... the ribosome where it recognizes the UAG amber codon and incorporates phosphoserine into the growing ...rEcoli XpS (Bacterial strain #192872) , and an optimized phosphoserine orthogonal translation system, pSerOTS-C1... C321.ΔA (Bacterial strain #68306) , with the optimized phosphoserine orthogonal translation system, SepOTSλ...Rogulina, S., & Rinehart, J. (2023). System‐wide optimization of an orthogonal translation system with enhanced...Isaacs, F. J., & Rinehart, J. (2015). A flexible codon in genomically recoded Escherichia coli permits ...Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion . FEBS...: SepRS adds a phosphoserine to tRNA^Sep with anticodon, CUA. Engineered EF-Tu binds the phosphoserine-charged...

  6. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...inhibit MMR PEmax - optimized PE2 enzyme containing RT optimized for human codons, additional nuclear ...PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding...

  7. Resolute Plasmid Collection

    Type
    Collection
    ... Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...

  10. AAV Molecular Tools

    Type
    Collection
    ...CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...which circumvents the problem of in-frame amber codons in scFvs A new expression vector facilitating production...

  12. Lentiviral Prep Service

    Type
    Collection
    ...Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

  13. Open Enzyme Collection

    Type
    Collection
    ... (Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...proteins in mammalian cells or other species by codon optimization of their sequence and eliminating interactions...Resnitzky et al., 1994). TRE3G promoter : also optimized into P TRE3GV for viral expression and P TRE3GS...Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the Tet-On system for regulated gene expression...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often referred...® protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory...

  16. Plasmids for Stem Cell Research

    Type
    Collection
    ...p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

  17. CRISPR Guide

    Type
    Collection
    ... an optimized gRNA for your application (see: Plan Your Experiment ). In addition to an optimized gRNA... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV... Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221_SLC1A3 SLC1A3...
Showing: 1 - 18 of 18 results