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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5 results for: dcas9 vp64 expression plasmid

Showing: 1 - 5 of 5 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP... dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with ...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator...with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...Addgene plasmids #61422 - #61424 were designed for transient transfection : 61422 : Expresses dCAS9-VP64 ...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase... SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...., Science 2013). Additionally, we have a plasmid expressing SpCas9 alone without sgRNA. Full references...nickase) + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized ...

  3. Plan Your Experiment

    Type
    Collection
    ... Reduce gene expression without permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional...transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be more effective than dCas9 alone for mammalian cell...activator (such as dCas9-VP64) Many different activator systems exist, including the multi-plasmid SAM system ...CRISPRa) Increase gene expression without permanently modifying the genome sequence dCas9 fusion with transcriptional...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ... your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...requires expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem...

  5. CRISPR Guide

    Type
    Collection
    ...system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold gRNA and additional...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent...biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...genomic DNA. dCas9 has also been used for genome-wide screens to activate or repress gene expression in mice...human cells. The simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single...
Showing: 1 - 5 of 5 results