CRISPR Guide
Type
Collection
...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...application, the donor DNA may be a single-stranded oligonucleotide, double-stranded linear DNA, or a double-stranded...-stranded DNA (dsDNA) plasmid. Small single-stranded DNA (ssDNA) oligos often work well for small edits...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...