Plan Your Experiment
Type
Collection
...or you can generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is...want to: Generate complete and permanent loss of gene expression or function (knockout)? Generate a specific...transfect expression plasmids encoding your Cas protein and gRNA, which you generally perform by chemical...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...vectors is that the packaging limit (how large of a DNA sequence you can actually package into your viral ...always required, and a mixed population can be sufficient for many experiments. A mixed population will contain... vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with smaller Cas...