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We narrowed to 3 results for: gfp donor template

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  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ... the genomic site and the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked... and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid...following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection...Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB...FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen...created using CRISPR and the donor plasmids containing homology arms and EGFP are available at Addgene. ...101 post for more information about HDR/repair template design. How to use CRISPR to tag your gene of ...

  2. CRISPR Guide

    Type
    Collection
    ...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA...(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion ...contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-...PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: ...luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to... recombinase-2A-EGFP-KASH 60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone...recombinase-2A-EGFP-KASH and an sgRNA. #60231 - AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR...
Showing: 1 - 3 of 3 results