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Showing: 1 - 4 of 4 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression ...systems, including Xenopus HA Epitope tag pcDNA3.1-HA or c-Flag pcDNA3 - Mammalian expression...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...gene with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6 translation for mammalian expression...Expression collection page Plant CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA...with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation... your protein to an epitope tag, such as Flag or HA, will allow you to easily identify your protein...

  2. Genetic Code Expansion

    Type
    Collection
    ...sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP... RF1 function removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 ...its cognate aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids...aminoacylate the orthogonal tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls....1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA synthetase...

  3. Cre-lox system

    Type
    Collection
    ...codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin Plant Birchler 140131 pAAV-EF1a-NCreV N-terminal...fluorescent labels that indicate if recombination has occurred, allowing for direct comparison of Cre+ ...optimize Cre expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special...rates of Cre-driven recombination. CREM (or Cre-M) has been engineered to contain an intron, preventing ...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-... MHC-G HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...MSTP084 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...associated protein C20orf188, TRRP4AP, TRUSS UBR1 ubiquitin protein ligase E3 component n-recognin 1 JBS, ...
Showing: 1 - 4 of 4 results