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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5 results for: hygr cassettes

Showing: 1 - 5 of 5 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) 71485 Mammalian none S. pyogenes Hygro Sherwood pMpGE_En01 71534 Other...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369...gRNA module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or ...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized SpCas9, driven...

  3. Lentivirus Plasmids

    Type
    Collection
    ...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730... plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned ...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...plasmid with puro resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning ...expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression...expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions...

  4. Cre-lox system

    Type
    Collection
    ...p252 pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green 8408 p255 pPr-CREM/Ins...Ins/CMV-STOP-luc CREM and CMV-STOP-luc cassette, separated by insulator probasin Mammalian Green 11543...Sauer 12168 pMB80 (R26-CreER) Cre-ERT2 with loxp cassette; Targeting vector for Rosa26 locus Mammalian Jacks...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...activates gene of interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration...pPNT6 Cre activates gene of interest, removes Neo cassette. Used in gene targeting. Mouse Targeting Shivdasani...pMSCVpuro-Cre Cre 5'LTR Retroviral Ge 34565 pMSCVhygro-Cre Cre and HygroR coexpression Retroviral Ge 34568 WZLneo-Cre...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...create stable cell lines in which the expression cassette of interest is incorporated into the host...puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant... (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more empty backbones...Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA...
Showing: 1 - 5 of 5 results