We narrowed to 3 results for: hygr cassettes

...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized SpCas9, driven...

...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk Parijs ...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan Kissler... plasmid with a chimeric 5’LTR. Any expression cassette (promoter and gene of interest) can be cloned ...interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). Find...with puromycin selection. See plasmid 24150 for hygromycin selection. Bob Weinberg 10878 pLKO.1 – TRC cloning...with puromycin selection. Ie-Ming Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression...expression, gateway plasmid, and constitutive hygromycin selection. See Shin et al. (2006) for additional variants...

...create stable cell lines in which the expression cassette of interest is incorporated into the host...puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant... (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more empty backbones...Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA...