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We narrowed to 9 results for: lentiviral infection

Showing: 1 - 9 of 9 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...entry and infection. This approach allows researchers to safely study mechanisms of infection and to determine... measuring infectivity and neutralizing activity are listed below: pBOBI-FLuc - Lentiviral reporter vector...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood but are critical for ...Pseudotyped virus particles can then be used to infect permissive cells that express ACE2, the cellular...methods of neutralizing the virus or reducing its infective potential. The following types of viral particles...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...

  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile ...Capacity Virus Size Cells Infected Host Genome Integration Immune Response Lentiviral Stable RNA < 8 kb 80–...relatively high transduction efficiency and only infect dividing cells. They integrate permanently into... to produce viral vectors. A) Wild-type viruses infect cells in an organism and use the host cellular ...

  3. Luciferase Plasmid Collection

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    Collection
    ... are infected with virus Christopher Buck 98580 pLX304 Luciferase-V5 blast Firefly CMV Lentiviral expression...interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse...Renilla Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly, Renilla... can be used to monitor transfection or viral infection efficiency, to study changes in cell physiology...21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase Eric Campeau...

  4. Lentivirus Plasmids

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    Collection
    ...target cell upon infection. When lentivirus is used for research, it is the lentiviral genome that encodes...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...table lists lentiviral transfer plasmids that can be used with 2nd and 3rd generation lentivirus technologies...

  5. Retrovirus Plasmids

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    Collection
    ...Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...relatively high amounts of DNA (up to ∼8 kb), and infection results in long-term transgene expression. Some...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-... Thy1.1 - Lentiviral shRNA expression Gentamicin Varies pJL-TRBO - An improved agroinfection-compatible...N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway...for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing and nondividing ...

  7. Kazuhiro Oka Lentiviral Vectors

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    ...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...For more information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral... in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral Plasmid ...vectors. Download the Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor... Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...

  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...kill cells that have been infected by a foreign pathogen. They detect infected cells via receptors such...construct complex lentiviruses for modification of mammalian cells with a single viral infection from the lab...pathogens each day. Their ability to protect against infection depends on two systems that work in concert: the... the innate and adaptive immune systems. Upon infection, the immune system triggers several processes ...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...destruction of pathogens. Over the course of an infection, adaptive immune cells will expand and give rise...

  9. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...
Showing: 1 - 9 of 9 results