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Showing: 1 - 8 of 8 results
  1. TALEN Plasmids and Kits

    Type
    Collection
    ...rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3...
  2. Luciferase Plasmid Collection

    Type
    Collection
    ...Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla... processes, biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created ...Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...fusions using Gateway cloning Mikko Taipale 87065 pcDNA3.1-ccdB-Renilla Renilla Creation of C-terminal Renilla...fusions using Gateway cloning Mikko Taipale 87066 pcDNA3.1-ccdB-Firefly Firefly Creation of C-terminal Firefly...fusions using Gateway cloning Mikko Taipale 87068 pcDNA3.1-Renilla-ccdB Renilla Creation of N-terminal Renilla...
  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 ...Aaron Gitler 29609 pcDNA 3.2-FUS-1-526aa-V5 FUS V5 CMV ALS Aaron Gitler 29610 pcDNA 3.2-FUS-1-269aa-V5...Aaron Gitler 29611 pcDNA 3.2-FUS-1-373aa-V5 FUS V5 CMV ALS Aaron Gitler 29612 pcDNA 3.2-FUS-1-413aa-V5...Lindquist 14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A...Mombaerts 15938 pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D...Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's Harm Kampinga 19484 pcDNA5/FRT/TO GFP HSPA1L...Kampinga 19511 pcDNA5/FRT/TO V5 HSPA1L HSPA1L V5 CMV Parkinson's Harm Kampinga 19538 pcDNA5/FRT/TO HIS HSPA1L...
  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pSCFP3A-C1 - Mammalian Expression mTurquoise2 434 474 28 3.1 Monomer (A206K) pmTurquoise2-C1 - Mammalian Expression...Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian...Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Clover 505 515 84 6.1 30 min Prone to dimerization pcDNA3-Clover - Mammalian Expression pFA6a-link-yoClover-Kan...Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz 514 527 57 Prone...Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression...mRuby2 559 600 43 5.3 150 min Prone to dimerization pcDNA3-mRuby2 - Mammalian Expression pFA6a-link-yomRuby2...
  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein 11546...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...
  6. Cre-lox system

    Type
    Collection
    ...122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F...Binding Domain (EBD) Tdh4 Yeast van Leeuwen 65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular...
  7. Genetic Code Expansion

    Type
    Collection
    ...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...
  8. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...human IRF-1 promoter. 98041 Sa-dCas9-NLS-3xFLAG/pcDNA3.1 Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells...2018 May 5;499(2):291-298. doi: 10.1016/j.bbrc.2018.03.150. PubMed . enChIP systems using different CRISPR...
Showing: 1 - 8 of 8 results