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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 10 results for: pcDNA3

Showing: 1 - 10 of 10 results

  1. Arf GTPase Family

    Type
    Collection
    ...pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...(pET), Mammalian (pcDNA3) GTPase Arfrp1 10139 201 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase ... (pET), Mammalian (pcDNA3) GAP Arfgap2 84364 521 Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap3 26286...pGEX4T), Mammalian (pcDNA3) GAP ELMOD2 255550 293 Bacterial (pGEX4T), Mammalian (pcDNA3) GAP ELMOD3 84173...GTPase Arf6 382 175 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian... EGFP 488 507 34 6 25 min Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...(StayGold-E138D) 497 504 126 4.6 14 min Monomer pcDNA3-10His-mStayGold (E138D) - Mammalian Expression ...Clover 505 515 84 6.1 30 min Prone to dimerization pcDNA3-Clover - Mammalian Expression pFA6a-link-yoClover-Kan...Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz 514 527 57 Prone... Expression mRuby2 559 600 43 5.3 2.5 h Monomer pcDNA3-mRuby2 - Mammalian Expression pFA6a-link-yomRuby2...Bacterial Expression mRFP1 584 607 13 4.5 1 h Monomer pcDNA3-mRFP - Mammalian Expression pMXs-mRFP1 - Mammalian...

  3. Fluorescent Proteins: FRET

    Type
    Collection
    ...mCherry2-N1 , pcDNA3.1 CMV mCherry-eGFP BGH Clover mRuby2 505 0.76 600 113,000 0.38 6.3 4.5 pcDNA3-Clover ,... , pcDNA3-mRuby2 , pcDNA3.1-Clover-mRuby2 mClover3 mRuby3 506 0.78 592 128,000 0.45 6.4 4.9 pNCS-mClover3...0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET-10 SYFP2 mScarlet3...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression ...plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression pENTR4-myc-nuc...C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3 or 3xHA-miniTurbo-NLS_pCDNA3 - Expresses 3xHA-tagged TurboID...expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells... including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian expression vector with N-terminal...plasmids with Neomycin selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Lindquist 14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A...Mombaerts 15938 pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D...Arrowsmith 65106 pcDNA3-ADAM10-HA ADAM10 HA CMV Alzheimer's Axel Ullrich 65107 pcDNA3-Delta(Pro-MP)ADAM10...Parkinson's Dori Woods 197146 pcDNA3-ApoE2 APOE CMV Alzheimer's Bradley Hyman 197147 pcDNA3-ApoE3 APOE CMV Alzheimer's...E320A) pcDNA3 CACNA1A CMV Spinocerebellar ataxia 6 Annette Dolphin 206125 Cav2.1 EIVA (E1707A) pcDNA3 CACNA1A...HTRA2 GFP CMV Parkinson's L. Miguel Martins 14124 pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. ...HTRA2 Flag CMV Parkinson's L. Miguel Martins 15940 pcDNA3-HtrA2-FLAG S400D HTRA2 Flag CMV Parkinson's L. ...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...firefly luciferase Eric Campeau 18964 Luciferase-pcDNA3 Firefly CMV Mammalian expression of firefly luciferase...Renilla luciferase Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern...upstream of IRES-firefly luciferase. Bryan Welm 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression of Nanoluc...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein 11546...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...

  9. Genetic Code Expansion

    Type
    Collection
    ...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182652 pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF) PylRS M. mazei...mazei Mammalian TAG Ivana Nikić-Spiegel 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3...
Showing: 1 - 10 of 10 results