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  1. Mammalian RNAi Tools

    Type
    Collection
    ...information on shRNA design and delivery. Constitutive Lentiviral shRNA Lentiviral vectors for constitutive...Trono Cre-regulated Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression ID Plasmid...Jacks Tet-Regulated Lentiviral shRNA Lentiviral vectors for Tet-regulated shRNA expression ID Plasmid ...constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended...David Root 1864 pLKO.1 ‐ scrambled shRNA Negative control vector containing scrambled shRNA David Sabatini...8452 pMKO.1 puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Mammalian pLKO.1 - TRC cloning vector - Lentiviral shRNA expression Tet-pLKO-puro - ...Hygromycin Mammalian pLKO.1 hygro - Lentiviral shRNA expression Tet-pLKO-puro - ...Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral shRNA expression pLenti...activity shRNA/RNAi For gene silencing experiments pLKO.1 - TRC cloning vector - Lentiviral...Neomycin (G418) Mammalian Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pLenti CMV ...Tet-inducible lentiviral shRNA expression pLenti CMV Hygro DEST - Lentiviral Gateway destination... bacterial expression vector (see tagged expression vectors from the SGC) pPro18...

  3. Lentivirus Plasmids

    Type
    Collection
    ...expression of shRNAs; addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression...Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance....resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a ...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...PI 21916 Tet-pLKO-neo 3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection...11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene or shRNA; see article for detailed...Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA; selection cassette in...third-generation rtTA On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression; see article for additional...pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation of shRNA efficacy using fluorescence;... (w756-1) Lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter...Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background ...

  5. Cre-lox system

    Type
    Collection
    ...Mammalian Sofroniew 25997 LV-Cre pLKO.1 Cre and shRNA coexpression CMV Lentiviral Fuchs 26646 pCAG-Cre-IRES2...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...Cre-dependent shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...cassette; Targeting vector for Rosa26 locus Mammalian Jacks 12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 ...17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 ...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian Luo...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [...leukoencephalopathy David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410 px335 Slc1a3 nickase 1 SLC1A3 HA U6...HA-KIF5A KIF5A HA CMV ALS Rosalind Segal 167294 pLKO.1 puro_shTBK1_3185 TBK1 ALS Agnel Sfeir 169375 pET3d-SF-DCTN1...Cookson 13321 pGEX5X.1 PINK1 WT PINK1 GST tac Parkinson's Mark Cookson 13322 pGEX5X.1 PINK1 KD PINK1 GST...
Showing: 1 - 6 of 6 results