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Showing: 1 - 4 of 4 results
  1. Mammalian RNAi Tools

    Type
    Collection
    ...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral backbone, with some...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized...Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing...
  2. Cre-lox system

    Type
    Collection
    ...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...GFP and pSico PGK puro Lentiviral Jacks 11579 pSicoR Cre turns off shRNA expression, removal of EGFP; See...cassette; Targeting vector for Rosa26 locus Mammalian Jacks 12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 ...17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 ...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian Luo...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...Cre-ERT2;Targeting vector for Fos locus Mammalian Luo 46389 pArcCreER Cre-ERT2;Targeting vector for Arc locus...
  3. Lentivirus Plasmids

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    Collection
    ...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...cell upon infection. When lentivirus is used for research, it is the lentiviral genome that encodes genetic...table lists lentiviral transfer plasmids that can be used with 2nd and 3rd generation lentivirus technologies...
  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 12165 pSico Dnmt1 DNMT1 GFP CMV Hereditary sensory neuropathy type IE Tyler Jacks 12166 pSicoR Dnmt1 ...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...
Showing: 1 - 4 of 4 results