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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8 results for: puromycin selection marker

Showing: 1 - 8 of 8 results

  1. Lentiviral Prep Service

    Type
    Collection
    ... Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178 Human... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... with G418 selection Find nematode transgenesis plasmids with Neomycin selection Puromycin Mammalian Tet-pLKO-puro...function, selectable markers, and more. Plasmid...site (MCS), an inducible promoter, and/or a selectable marker. They are frequently used in molecular biology...Genome Modification, mRNA Regulation, and More Selectable Markers Species-Specific Expression If you want to...retroviral gene expression with GFP or mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral...the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of your delivery method, it's ...Thus, many plasmids have markers on them so that you can find or select for only the cells that received...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication... epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9. ID...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG...

  4. Lentivirus Plasmids

    Type
    Collection
    ...inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico...shRNA empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro...or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau ...-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...expression of shRNA with puromycin selection. See Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri...design of inducible shRNA expression, with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid...article ( Kowarz et al., 2015 ) for additional selection markers. rtTA-Advanced Tight TRE Eric Kowarz 96930...expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521...vector with blasticidin selection. See Plasmid #26730 for hygromycin selection. rtTA3 Eric Campeau 128061... Plasmid #85972 for hygromycin selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral...vector for repressible expression with blasticidin selection. *Note: this vector contains an insert (MAT2A)...

  6. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker or cloning method...Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6...

  7. Retrovirus Plasmids

    Type
    Collection
    ...) MSCV For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs in which... sgRNA and SpCas9, but does not contain markers for selection 1 . Other constructs may be utilized, such...Screening CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a...conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject...initial screen ensures that the correct clone was selected and reproducibility of results. To evaluate the...
Showing: 1 - 8 of 8 results