Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 6 results for: shRNA construct

Showing: 1 - 6 of 6 results

  1. Lentivirus Plasmids

    Type
    Collection
    ...expression of shRNAs; addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression...the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging...GP. Good for work in human MSCs. Use packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid ...10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning...cloning Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1...

  2. Cre-lox system

    Type
    Collection
    ...Cre-dependent shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression...switch CMV Mammalian Livet loxP (floxed) Constructs These constructs allow for Cre-regulated gene expression...Depending on the construct, Cre may activate or repress gene expression. Common construct types include:... expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest, Genes...Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References...recombination principles, scientists have developed constructs to activate/inactivate genes when Cre is present...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors for cloning in shRNAs, including plasmids...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP...a different genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope ...delivery, genome modification, reporter assays, shRNA expression, transgenics, and more. See the next ...retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones made by Addgene ...uses such as genome modification, reporter assays, shRNA expression, transgenics, and more. Element Details...Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pnCasPA-BEC - A cytidine deaminase-mediated...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ... pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid...Promoters Blog: Repressible Promoters Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection ...Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control expression of your...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for...plasmid for easier cloning and design of inducible shRNA expression, with puromycin selection. See Plasmid... al., 2011 ) for additional tookit plasmids for shRNA or gene expression. rtTA-Advanced H1-2O2 Stephen...vector for inducible expression of transgene and/or shRNA. See article ( Szulc et al., 2006 ) for Tet-Off ...

  5. Retrovirus Plasmids

    Type
    Collection
    ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro ...cytoplasm Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct for miRNA expression Chen 52114 pMSCV-IRES-mCherry...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...leukoencephalopathy David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...28203 tdp43-EGFP construct10 TARDBP GFP CMV ALS Zuoshang Xu 28204 tdp43-EGFP construct11 TARDBP GFP CMV ...Josef Kittler 163858 VPS35 shRNA1 VPS35 Parkinson's Lei Lu 163859 VPS35 shRNA2 VPS35 Parkinson's Lei Lu ...
Showing: 1 - 6 of 6 results