We narrowed to 7 results for: shRNA construct

...(Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See Ventura et al. ...the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — ...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid...10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning...cloning. David Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for...21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. See plasmid 21916 for ...

...deleted. shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression... expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest, Genes...regulate your gene of interest. Depending on the construct, gene expression may be activated or repressed...

...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid 8452...William Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct for miRNA expression. Chang-Zheng Chen 52114 pMSCV-IRES-mCherry...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional...

...basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors for cloning in shRNAs, including plasmids...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP...a different genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope ...delivery, genome modification, reporter assays, shRNA expression, transgenics, and more. See the next ...retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones made by Addgene ...uses such as genome modification, reporter assays, shRNA expression, transgenics, and more. Element Details...Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pnCasPA-BEC - A cytidine deaminase-mediated...

... pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid...Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control expression of your...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for...plasmid for easier cloning and design of inducible shRNA expression, with puromycin selection. See Plasmid... al., 2011 ) for additional tookit plasmids for shRNA or gene expression. rtTA-Advanced H1-2O2 Stephen...vector for inducible expression of transgene and/or shRNA. See article ( Szulc et al., 2006 ) for Tet-Off ...Return to Top Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetracycline...

...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing...introns Anthony Leung Return to Top Expression Constructs Luciferase driven by a strong constitutive promoter...luciferase). Feng Zhang Return to Top Reporter Constructs Already know what gene or regulatory element ...reporter for? Browse or use the search box to find a construct that contains your regulatory element of interest...rapamycin-inducible interaction. nLuc and cLuc : Constructs for split firefly luciferase complementation ...and Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag...

...Parkinson's David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1) NR4A2 GFP CMV Parkinson's Glenn Schafe 233274 pAAV-CMV-GFP-pA U6 shRNA (Rat ...ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...