... and the B strain. K-12 has led to the common lab strains MG1655 and its derivatives DH5alpha and DH10b...DH10b (also known as TOP10) among others, while the B strain gave rise to BL21 and its derivatives. We've...Genotype BL21 Invitrogen; New England BioLabs E. coli B F dcm ompT hsdS(rB mB) gal ccdB Survival Invitrogen...lacIqZDeltaM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697...Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Hygromycin B 200 mg/mL 200 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin...Uracil Single Letter Code: Ambiguous bases Nucleobase B C, G, or T D A, G, or T H A, C, or T K G or T M A ...lacY1 proA2 rpsL20(StrR) xyl5 lambda- leu mtl1 DH5alpha Invitrogen F- Phi80lacZDeltaM15 Delta(lacZYA-argF...

...region of interest (deletions or small insertions) or (B) span the genome-insert boundary (insertions only)... 11315. PMID: 26066060 Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J.... Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically...