... pBABE vectors, forward primer pGEX 5' GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward... C (UbC) promoter, forward primer IRES-F TGGCTCTCCTCAAGCGTATT 3' end of IRES, forward primer IRES-R CCTCACATTGCCAAAAGACG...stem cell virus, forward primer MSCV-rev CAGCGGGGCTGCTAAAGCGCATGC Murine stem cell virus, reverse primer...Biosciences) Myc tag, forward primer Neo-F CGTTGGCTACCCGTGATATT 3' end of neomycin resistance gene, forward... in pENTR vector, forward primer pENTR-R ATGGCTCATAACACCCCTTG 3' of attL2 in pENTR vector, reverse primer...in pGEX vectors, reverse primer pGEX 5' GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward...Renilla luciferase, forward primer RVprimer3 CTAGCAAAATAGGCTGTCCC (Promega) 5' of MCS in pGL3 vector, forward...