Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 6 results for: Gateway entry

Showing: 1 - 6 of 6 results

  1. Molecular Cloning Techniques

    Type
    Guide
    ...completed via Gateway cloning. Addgene's collection contains thousands of Gateway donor , Gateway entry , and ...backbone. Created with BioRender.com. Gateway Recombination Cloning Gateway cloning is a recombination-based...via a proprietary BP clonase, creating an entry clone. The entry clone now has recombined attL sites flanking...made an entry vector containing your DNA of interest (or obtained one of the thousands of entry vectors...experimental goal. Although Gateway cloning is a simple and efficient cloning method, Gateway vectors and recombination...conditions. Read more in our Gateway Cloning blog post . Figure 2: Summary of Gateway cloning. Created with ...further reducing ligation efficiency. TOPO-ready Gateway entry plasmids are also available, allowing for rapid...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...compatible with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or...the viral particles is removed and stored or centrifuged to concentrate. Crude or concentrated virus can...gamma-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturing. Molecular Therapy...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...compatible with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...Blood-brain barrier (BBB) Layer of cells that prevent the entry of toxins and pathogens from the blood to the CNS...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or...Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR...

  4. Adenovirus Guide

    Type
    Guide
    ...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...compatible with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...compatible with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or...containing the viral vectors is removed and stored or centrifuged to concentrate. You can use crude or concentrated...

  6. Molecular Biology Reference

    Type
    Guide
    ...fragment. Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. Empty Backbones...
Showing: 1 - 6 of 6 results