We narrowed to 4 results for: PCR-amplified REST

1. Molecular Cloning Techniques

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   Guide

   ...is also easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to...similar to restriction enzyme cloning. To do this, your DNA fragment must first be amplified with specific... rapid cloning of PCR products into donor plasmids without the need for restriction enzyme cloning. Read...the PCR primers for the insert, based on the destination vector sequence and choice of restriction site... Protocols Plasmid cloning by restriction digest Plasmid cloning by PCR DNA ligation Gibson assembly Ligation...molecular cloning, scientists can amplify and manipulate genes of interest and then insert them into plasmids...naturally leaves a single A overhang on the 3' end of PCR products. The complementary T comes from a pre-cut...

2. Guide to Using Pooled Libraries

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   ...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their cDNA...see if it should be amplified before you conduct your screen. If you need to amplify the library, please...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens... 175,000 different gRNA-containing plasmids). Amplifying and Using the Library Once you receive your library...reproducibility. Figure 1: Simplified flow chart for amplifying and using a pooled library. The process diverges...negative screens follow the same basic process: Amplify the library (electroporation and maxiprep). If ... Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read ...

3. Molecular Biology Reference

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   ... sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers ... for the ability of the plasmid to be copied (amplified) by bacteria. Antibiotic Resistance Gene Allows...pieces, labeled with a short DNA barcode, and amplified. These DNA fragments are attached to a glass slide...separated. These attached DNA templates are then amplified again, producing ~1,000 copies of each template...plasmid using bacteria, which take up plasmids and amplify them during cell division. Because bacteria are...Additionally, the restriction enzyme site(s) allow for the cloning of a DNA fragment of interest into the plasmid...contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzyme digestion...

4. CRISPR Guide

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   ...enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific sequence of DNA...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by...conditions: The sequence is unique compared to the rest of the genome The target is present immediately ...verify that the composition of your amplified library matches that of the original library. Next, you’... copies of the aptamer improves stability and amplifies the signal for better imaging of genomic loci,...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates... Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20...