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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: aav gfp cre

Showing: 1 - 3 of 3 results

  1. Adenovirus Guide

    Type
    Guide
    ...for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses ...viruses that belong to the Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations...how they got their name, but they are not related. AAVs require the presence of adenoviral genes E1, E4,...contamination have been developed, such as using the Cre-lox and Flp-FRT recombination systems to excise the...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...

  2. Molecular Biology Reference

    Type
    Guide
    ...use these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...expanding viral service offers select ready-made AAV and lentiviral particles. Visit our viral service...study DNA fragments of interest, such as genes. Creation of recombinant DNA Molecular Cloning Plasmids ... With current cloning technology, it is easy to create and modify plasmids containing the genetic element...vector can also include an enhancer sequence which increases the amount of protein or RNA produced. Expression...used in place of ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise...

  3. CRISPR Guide

    Type
    Guide
    .... Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...used for large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in... GAT; increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease...others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...
Showing: 1 - 3 of 3 results