Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 3 results for: aav vectors expressing gfp

Showing: 1 - 3 of 3 results

  1. Adenovirus Guide

    Type
    Guide
    ...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...shuttle/transfer vectors for recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping...shuttle/transfer vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant...Uses of Adenoviral Vectors Although recombinant adeno-associated viral (rAAV) vectors are often the preferred...

  2. Molecular Biology Reference

    Type
    Guide
    ...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors...amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...for the transcription process to stop. An expression vector can also include an enhancer sequence which...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...

  3. CRISPR Guide

    Type
    Guide
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...
Showing: 1 - 3 of 3 results