Modular Cloning Guide
Type
Guide
... of Type IIS restriction enzymes BsaI and BpiI/BbsI to cut outside their recognition site, leaving DNA...with a standard overhang sequence at the restriction cut site, multiple modules with complementary overhangs...ligated in a single step resulting in a 4-bp fusion site in between. MoClo can be used for a variety of applications—like...sequences, terminators, etc.) flanked by fusion sites into individual Level 0 plasmids, or choose from...about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and ...terminators, 4 nonfunctional "dummy" parts, 34 backbones, and 6 destination vectors for use in E. coli ...including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome editing in monocots ...