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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: dcas9, grna

Showing: 1 - 3 of 3 results

  1. CRISPR Guide

    Type
    Guide
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent... specified by a particular gRNA. Epitope tag(s) are fused to dCas9 or gRNA for efficient purification ...CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator with a gRNA to increase transcription of a target...CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA to repress/decrease transcription... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...

  2. Plan Your Experiment

    Type
    Guide
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be...edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit. Science... system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different ... proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...your gRNA is the balance between on-target and off-target activity. In a perfect world, your gRNA sequence...

  3. Modular Cloning Guide

    Type
    Guide
    ...expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use...plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome editing in monocots...
Showing: 1 - 3 of 3 results