We narrowed to 2 results for: dna oligonucleotides

...archaea, cleave double-stranded DNA (dsDNA) at specific sequence sites in the DNA. In restriction cloning, scientists...ends in the DNA fragments. Given its prevalence, the vast majority of plasmids used for DNA cloning and...flanking your DNA fragment of interest. Now that you have made an entry vector containing your DNA of interest... to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity... has difficulty assembling DNA fragments with repetitive sequences and DNA that ends in sequences that...into yeast the fragments of DNA to be fused along with custom synthesized DNA oligos that span each junction...is the ability to perform oligonucleotide stitching, in which pieces of DNA that share no homology ends...

...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...application, the donor DNA may be a single-stranded oligonucleotide, double-stranded linear DNA, or a double-stranded...-stranded DNA (dsDNA) plasmid. Small single-stranded DNA (ssDNA) oligos often work well for small edits...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...