...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...