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  1. Modular Cloning Guide

    Type
    Guide
    ...use with the Golden Gate TALEN and TAL Effector Kit (Voytas and Bogdanove). Platinum Gate TALEN Kit Genome... for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather than two. Golden GATEway...Wittbrodt A toolkit that combines Golden Gate and Multisite Gateway cloning. This system was specifically...Cloning Applications and Kits Blog: Plasmids 101 Golden Gate Cloning Synthetic Biology Plasmids Modular Cloning... (Weber et al., 2011). This method, based on Golden Gate plasmid assembly, exploits the ability of Type...eight transcription units. Yarrowia lipolytica Golden Gate tool kit Yeast Expression Tristan Rossignol 64...for individual subunits are first assembled by Golden Gate cloning and then combined by Gibson Assembly ...
  2. Molecular Cloning Techniques

    Type
    Guide
    ...assembly. Created with BioRender.com. Golden Gate and MoClo Golden Gate and Modular Cloning (MoClo) take advantage...MoClo and Golden Gate cloning kits for your next cloning project. Read more in our Golden Gate Cloning ...Cloning Techniques Restriction Enzyme Gateway TOPO Gibson Golden Gate & MoClo Ligation Independent Cloning...Cloning blog post . Figure 5: Summary of Golden Gate cloning. Created with BioRender.com. Ligation Independent...Gate Cloning Tips and Tricks for Using Golden Gate Modular Cloning Plasmids 101: Sequence and Ligation Independent...101: Gateway Cloning Plasmids 101: TOPO Cloning Plasmids 101: Gibson Assembly Plasmids 101: Golden Gate...completed via Gateway cloning. Addgene's collection contains thousands of Gateway donor , Gateway entry , and...
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