We narrowed to 2 results for: golden gate assembly

...subunits are first assembled by Golden Gate cloning and then combined by Gibson Assembly into acceptor vectors...of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs. FusX TALEN Assembly System Kit Genome Engineering...Ekker 336 plasmids of pre-assembled pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts...et al., 2011). This method, based on Golden Gate plasmid assembly, exploits the ability of Type IIS restriction...lipolytica Golden Gate tool kit Yeast Expression Tristan Rossignol 64 plasmids to assemble and integrate...plasmids that allow for the assembly of cellular circuits using Golden Gate-based cloning methods for use...use with the Golden Gate TALEN and TAL Effector Kit (Voytas and Bogdanove). Platinum Gate TALEN Kit Genome...

.... Read more in our Golden Gate Cloning blog post . Figure 5: Summary of Golden Gate cloning. Created with...Gibson Assembly blog post . Figure 4: Summary of Gibson assembly. Created with BioRender.com. Golden Gate...Cloning Techniques Restriction Enzyme Gateway TOPO Gibson Golden Gate & MoClo Ligation Independent Cloning...101: Gateway Cloning Plasmids 101: TOPO Cloning Plasmids 101: Gibson Assembly Plasmids 101: Golden Gate... Created with BioRender.com. Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more commonly...Gate and MoClo Golden Gate and Modular Cloning (MoClo) take advantage of the unique properties of type IIS... to assemble before you get started. Check out Addgene's website for easy-to-use MoClo and Golden Gate...