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We narrowed to 4 results for: lentiviral backbone

Showing: 1 - 4 of 4 results

  1. Guide to Using Pooled Libraries

    Type
    Guide
    ... Plasmids within a given library have the same backbone, but they express or target different genes. Some...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

  2. Molecular Biology Reference

    Type
    Guide
    ...as from a chromosome to a plasmid or vector DNA backbone. These tools are essential to the field of recombinant...of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can...unique and not located elsewhere in the plasmid backbone. In expression plasmids, the MCS is often located...entry vectors and TOPO cloning vectors. Empty Backbones , Cloning Kits Expression Plasmids Used for gene...promoter is used to initiate transcription. Empty Backbones Gene Knock-down Plasmids Used for reducing the...plasmids to create viral particles (most commonly lentiviral, retroviral, AAV, or adenoviral particles) that...primers reference page Choosing your perfect plasmid backbone Plasmids 101 series on the Addgene blog Addgene...

  3. CRISPR Guide

    Type
    Guide
    ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading and discrimination...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...

  4. Science Guides

    Type
    Guide
    ... Plasmids within a given library have the same backbone, but they express or target different genes. Some...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...
Showing: 1 - 4 of 4 results