...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...