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We narrowed to 5 results for: lentivirus vector

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  1. Lentiviral Vector Guide

    Type
    Guide
    ...Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations Vector Production...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...for decades. Wild-type lentiviruses have been engineered into lentiviral vectors that can be safely used...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector Production...more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors The genome of ...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses and gamma-retroviruses fall under the Retroviridae...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...

  3. Plan Your Experiment

    Type
    Guide
    ... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized... exact cloning strategy will depend on the gRNA vector you have chosen, so it is best to review the protocol...

  4. Science Guides

    Type
    Guide
    ...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...

  5. CRISPR Guide

    Type
    Guide
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...
Showing: 1 - 5 of 5 results