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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2 results for: stem loop

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  1. CRISPR Guide

    Type
    Guide
    ...Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID... Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular...enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic edits...Cas9 (CRISPR Type II) systems, while AcrV inhibits Cas12a (CRISPR Type V) systems. There are rare cases...The two main classes of CAST systems are type I and type V-K. Type I systems use a type I Cas effector, ...), while type V-K systems use a type V-K Cas effector, Cas12k. No matter the system, the Cas effectors...-vector systems, in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which...

  2. Modular Cloning Guide

    Type
    Guide
    ...Biology Plasmids Modular Cloning (or MoClo) is a system for efficiently assembling multiple DNA parts into...assembling gene-editing tools, and more. The MoClo system consists of three sets of cloning vectors (Level...coding sequences, CRISPR parts GreenGate Cloning System Plant Expression Jan Lohmann 56 plasmids to create...Maizel 68 plasmids to extend the GreenGate Cloning System (Lohmann) with additional Level 0 modules and components... plasmids compatible with the GreenGate Cloning System (Lohmann) to create plasmids with multi-gene constructs...MoClo-YTK parts for use). Yeast GoldenBraid Cloning System and Toolkit Yeast Expression Elena Caro Bernat ...generating transcriptional units using the GoldenBraid system, for integration in S. cerevisiae . Easy-MISE Toolkit...
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