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We narrowed to 4 results for: yeast expression

Showing: 1 - 4 of 4 results

  1. Modular Cloning Guide

    Type
    Guide
    ...constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 ...uLoop Assembly Kit Other, Yeast Expression, Plant Expression, Bacterial expression Christopher Dupont 95 ...transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression Irina Borodina Plasmids for...Aspergillus species. MoClo Yeast Secretion and Display (YSD) Toolkit Yeast Expression Paul Young 34 plasmids...display of proteins of interest. Yeast Secrete and Detect Yeast Expression Sylvestre Marillonnet Plasmids...secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast Expression Tomáš...protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast Expression Brigitte...

  2. Cloning

    Type
    Guide
    ...protocol ) Back to Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very...them into plasmids for replication and protein expression. So how do scientists recombine DNA? There are...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...advantage of the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently ...accomplish this, you just need to introduce into the yeast the two (or more) fragments of DNA that you would...be set up to grow, transform and purify DNA from yeast. Back to Top...

  3. Molecular Biology Reference

    Type
    Guide
    ...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors... produced. Expression vectors can drive expression in various cell types (mammalian, yeast, bacterial,... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA... control of a bacterial promoter, and is thus expressed in the bacteria by bacterial transcriptional machinery...restriction enzymes digestion and ligation. In expression plasmids, the MCS is often located downstream...that when a gene is inserted within the MCS, its expression will be driven by the promoter. As a general ...

  4. CRISPR Guide

    Type
    Guide
    ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...Knockout libraries often target 5′ constitutively expressed exons, while activation and repression libraries...libraries are also available, such as those for yeast or drosophila . Although CRISPR has been less widely...with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying your screening...
Showing: 1 - 4 of 4 results