We narrowed to 40 results for: plasmid

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Lab protocol for transfection of HEK293 cells to produce recombinant antibody.

Use this protocol to transfect mammalian cells with your plasmid of interest.

Use this protocol to prepare LB agar plates with antibiotic in your lab.

Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol.

Description of Polymerase Chain Reaction with protocol, tips and FAQ

This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody.

Tips on designing primers for PCR

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely.

Protocol for Ligation Independent Cloning

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Lab protocol to determine purity and concentration of recombinant antibodies using ready-to-use biosafe Coomassie stain and ImageJ software.

This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.

This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose response.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.