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  1. CRISPR Library Amplification

    Type
    Protocol
    ... Qubit. Each different DNA measurement type can report slightly different concentrations of DNA. In our...our hands fluorescent dye methods tend to report lower values than Nanodrop, although most protocols have...electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4 cells or other...Micropulser, 0.1 cm ) 20 mL SOC recovery media (Lucigen, 80026-1) 8X LB Agar + Antibiotic 245 mm bioassay... 1-2 minutes. Critical Be careful not to rip or shred the agar. Do so by gentle spreading. Some spreaders...
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