Lentivirus ddPCR Titration
Type
Protocol
...dilution series outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where TU is transducing...the 96-well plate sealer by gently touching the screen. Preparation of the Master Mix Place a ddPCR plate...the plate sealer is not at temperature, touch the screen on the plate sealer to allow it to get to temperature... concentration of RRE positive droplets should decrease by a factor of 2 across the dilutions. RPP30 copies...below, the concentration of RRE positive droplets increases by a factor of ~2 as you progress from the higher...stays relatively even across samples (green). To increase the accuracy of the titer, calculate an average...