We narrowed to 2 results for: TUF;

...original pLKO.1-TRC cloning vector has a 1.9kb stuffer that is released by digestion with AgeI and EcoRI... into the AgeI and EcoRI sites in place of the stuffer. The AgeI site is destroyed in most cases (depending... a complete map of pLKO.1 containing the 1.9kb stuffer, visit www.addgene.org/10878/ . Description Vector...The pLKO.1-TRC cloning vector contains a 1.9kb stuffer that is released upon digestion with EcoRI and .... The vector is 8901 base pairs total, and the stuffer insert is shown in all capital letters. I.2. Recipes...

...from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic separation...at two points by the enzyme BsaI, removing the stuffer sequence and sacB counter-selection marker (see...