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We narrowed to 5 results for: aav vector plasmid

Showing: 1 - 5 of 5 results

  1. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. ... Protocols AAV Titration by qPCR Using SYBR Green Technology AAV Titration by qPCR Using SYBR Green Technology...also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction The AAV Titration protocol... calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation Size...Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...

  2. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols...Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction ...polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used by the ... that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification...spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis... the Video! Sequence Analysis Verify important plasmid features using sequence analysis RNA Extraction...

  3. AAV Production in HEK293 Cells

    Type
    Protocol
    ...of each plasmid needed to have a 1:1:1 molar ratio with 2 mg total DNA per CS5 Plasmid Plasmid Size (bp... Protocols AAV Production in HEK293T Cells AAV Production in HEK293T...HEK293T Cells You may also like... AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology...conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene ...molar ratio of each plasmid: 2000 μg / 24,961 bp = 0.08 μg/bp Therefore, for each plasmid we need: Sample ...adeno-associated virus (AAV) in HEK293 cells. Protocols...Introduction This protocol can be used to produce AAV from one Five Chambers Cell-Stack (CS5) (Link opens...

  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ...ddPCR) for lentivirus vector titration.... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration of...of Lentivirus Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence...Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus Guide Virus Blog Posts...droplet digital PCR (ddPCR) to titer lentivirus vectors. This protocol specifically uses primers and probes...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL...

  5. General Transfection

    Type
    Protocol
    ...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...protocol to transfect mammalian cells with your plasmid of interest. Protocols...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral ..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work...
Showing: 1 - 5 of 5 results