...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...polyethylenimine. Transfections allow for transient expression of a gene of interest in a target cell line and...determine what ratio gives the highest percentage of GFP positive cells. Refer to the table below for a possible...the cells 24–48 h before checking for protein expression. Sample Data Legend: Lenti-X 293T cells were ...here by the amount of green fluorescent protein expression (green in the right panels) with a limited effect...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work...