Virus Protocol - Generating Stable Cell Lines
Type
Protocol
...vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete: 10% v/v FBS...lentiCas9-Blast and then selected with 1 µg/mL blasticidin for 9 days. Single cells were then plated in...