...regularly to confirm transgene expression. As polyclonal populations of resistant cells start proliferating... the most desirable level of expression. Over time, transgene expression in a polyclonal population may...vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a...Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector...protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral...lentiviral vector. Unlike the short-term protein expression observed using transient transfection approaches... lentiviral vectors enables long-term protein expression studies. Moreover, repeating experiments in a...