Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...Vector Element U6 Human U6 promoter drives RNA Polymerase III transcription for generation of shRNA transcripts...because polyT is a termination signal for RNA polymerase III. Note that these were the prevailing guidelines...to adjust the sequencing conditions if the DNA polymerase has difficulty reading through the secondary ...toxic to some cell lines. In these cell lines, substitute protamine sulfate for polybrene. c. Add lentiviral...