... tutorial we will discuss how to move a cDNA from one plasmid to another. However, the same technique ...version of full-length cDNA you can find for YGOI is in a bacterial expression vector. Using subcloning...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will...correct orientation in the recipient plasmid. (You don't want to express the antisense version of your gene...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo ...material. We recommend 1.5-2μg of donor plasmid and 1μg of recipient plasmid. It is also critical that as much...re-circularization of the recipient plasmid. You should treat your digested recipient plasmid with a phosphatase prior...