Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
... scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing... 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free...want to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio that...TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing non-...Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal...Envelope plasmid for producing viral particles. Note: pLKO.1 can also be used with packaging plasmid pCMV-...the pLKO.1 vector, producing a final plasmid that expresses the shRNA of interest. C.1 Recommended Materials...