....1 – TRC control Negative control vector containing non-hairpin insert. pLKO.1 – scramble shRNA Negative...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles....Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal 21-mer... Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2...which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse ...genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community...lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct...