...A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast and then selected with...integrated lentiviral vector. Unlike the short-term protein expression observed using transient transfection...lines using lentiviral vectors enables long-term protein expression studies. Moreover, repeating experiments...prepare cell stocks and/or harvest to test for protein expression. Typically, cells transduced with lower...particles. Incubate the cells with the virus for 48–72 h. Gently aspirate the media from the cells. Add 1.5...formed by expansion of single cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid...