...ready-to-use biosafe Coomassie stain and ImageJ software....(Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample is separated by denaturing polyacrylamide...protein band intensities are measured using ImageJ software and a standard curve is generated. The ratio of...Plastic tray Gel imaging system ImageJ or similar software Reagents Purified recombinant antibody, 0.9–1.1...- can use commercial standard or validate an in-house standard Before Starting Warm the hot plate to 100...Link opens in a new window) or a similar photo software to determine the relative intensity of the protein...