...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator...likelihood of multiple integration events decreases as the dilution increases. Calculate the transduction units...cell lines and selection markers. Note this assay requires staining resistant colonies with crystal violet... remove cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel pipette...enough master mix for all samples). Each sample requires 15 μL of master mix. Pro-Tips Use a "Universal...95 uL 20X 200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution 4 20 uL Dil. 3 80 uL 5X 5000X Dilution ...indicates the presence of primer dimers which can increase background signal and alter the Ct values of your...