...were transduced with lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin for 9 days. Single cells... generated from A549 cells transduced with lentiCas9-Blast 1 . A549 cells were transduced (MOI = 37) and...control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid... and selected with 1 μg/mL blasticidin for 9 days. Single cells from a stable cell pool were then expanded...expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...

...were transduced with lentiCas9-Blast and then selected with 1 µg/mL blasticidin for 9 days. Single cells... by expansion of single cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid...mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a stable cell culture...Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete: 10% v/v FBS...