Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
Type
Protocol
...commercial kit Plasmid Cloning Protocols for constructing and analyzing your plasmids. Name Description...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...