...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...Purification Purify recombinant antibodies using Protein A or Protein G columns Watch the Video! Coomassie Purity...Watch the Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer...Video! Western Blot Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...